| 摘要 |
<p>PURPOSE:To obtain a protein combination for e. g. producing IgG2b-type antibody for detecting the toxic secondary metabolites of Mycota, by binding fumonisin B1 to a protein through a crosslinking agent such as maleimidobenzoyloxysuccinimide or glutaraldehyde. CONSTITUTION:A crosslinking agent such as maleimidobenzoyloxysuccinimide or glutaraldehyde is bound to fumonisin B1, and a protein such as hemocyanin or bovine serum albumin optionally reduced is then added to the product to carry out a reaction to obtain the objective hapten antigen as fumonisin B1- protein combination, where the fumonisin B1 has been bound to the protein through the crosslinking agent. Next, this combination is administered to an animal to immunize the animal, and antibody-productive cells are taken from the resultant animal and put to cell fusion with highly proliferative cells to prepare the objective hybridoma having IgG2b-type anti-fumonisin monoclonal antibody productivity, and this hybridoma is then cultured to obtain the objective anti-fumonisin monoclonal antibody.</p> |
