MATERIALS AND METHODS FOR THE IMMOBILIZATION OF BIOACTIVE SPECIES ONTO POLYMERIC SUBSTRATES

摘要

The present invention is directed to support members having surfaces that ar e rendered hydrophilic for use as a substrate for the immobilization of bioactive species thereon. The hydrophilic surfaces are chemically stable on the support member. The surfaces are also chemically variable to provide a variety of chemically functional groups for immobilization of bioactive species thereto. The surfaces comprise polymeric surfactants attached onto t he surfaces of a support member and covalently cross-linked thereon to form a first layer. Hydrophilic polymers are then attached to the first layer on th e support member to form a second layer thereon. The second layer is used to enhance the hydrophilicity of a support member as well as to provide a substrate for immobilizing bioactive species thereto. Methods for forming th e first and second layers are also provided. In addition, methods for attachin g bioactive substances to the hydrophilic polymers are provided.