摘要 |
The invention relates to the field of minimal residual disease (MRD) diagnostics, which is progressively more applied for the evaluation of treatment effectiveness in patients with a hematological malignancy, such as B-cell precursor acute lymphoblastic leukemia (BCP-ALL), B-cell chronic lymphocytic leukemia (B-CLL), and multiple myeloma (MM). Provided are unique reagent compositions with carefully selected and thoroughly tested combinations of antibodies, for ≧8-color flow cytometric stainings as well as for 10-color and 12-color flow cytometric stainings, which can reach sensitivities of at least 10−4, even down to 10−5. Also provided are diagnostic kits and methods for detecting MRD. |
申请人 |
Erasmus University Medical Center Rotterdam |
发明人 |
VAN DONGEN, Jacobus Johannes Maria;VAN DER VELDEN, Vincent Henricus Johannes;BÖTTCHER, Sebastian;LANGERAK, Anthonie Willem;SZCZEPANSKI, Tomasz;RITGEN, Matthias;ORFAO DE MATOS CORREIA E VALE, José Alberto;FLORES MONTERO, Juan Alejandro;ALMEIDA PARRA, Julia Maria;MEJSTRÃ�KOVÃ�, Ester;MONTEIRO DA SILVA LUCIO, Paulo Jorge |