NOVEL PLANT-DERIVED MAP KINASE KINASE

摘要

A mitogen-activated protein (MAP) kinase kinase gene, tMEK2, was isolated fr om tomato cv. Bonny Best. By mutagenesis, a permanently-active variant, tMEK2MU T, was created. Both wild type tMEK2 and mutant tMEK2MUT were driven by a strong constitutive promoter, tCUP.DELTA., in a tomato protoplast transient expression system. Pathogenesis-related genes, PR1b1 and PR3, and a wound-inducible gene, ER5, were activated by tMEK2MUT expression revealing the convergence of the signal transduction pathways for pathogen attack and mechanical stress at the level of MAPKK. Activation of biotic and abiotic stress response genes downstream of tMEK2 occurred through divergent pathways involving at least two classes of mitogen-activated prote in kinase. This study shows that tMEK2 may play an important role in the interaction of signal transduction pathways that mediate responses to both biotic (eg disease) and abiotic (wound responsiveness) stresses.