发明名称 Mammalian model for diabetes
摘要 An object of the present invention is to provide a model mammal for diabetes onset, being useful for elucidating the onset mechanism of diabetes caused by a blockage of signal transduction from insulin, and for the development of a remedy for said diabetes, and to provide a screening method of a remedy for said diabetes. A diabetic-prone transgenic mouse is prepared in a process comprising the steps of: a transgene that contains a Meg1/Grb10 gene, an imprinted gene exhibiting maternal expression, or a human GRB10 gene in the downstream of a chicken beta-actin promoter and in the upstream of a rabbit beta-globin poly A is constructed, and subsequently the transgene is microinjected into a male proneucleus of a mouse fertilized egg; thus obtained egg cell is cultured and then transplanted into an oviduct of a pseudopregnant female mouse; after rearing up the recipient animal, baby mice that have the above-mentioned cDNA are selected from the mice born from the recipient animal.
申请公布号 US7271311(B2) 申请公布日期 2007.09.18
申请号 US20020252111 申请日期 2002.09.23
申请人 JAPAN SCIENCE AND TECHNOLOGY AGENCY 发明人 ISHINO FUMITOSHI;MIYOSHI NAOKI;ISHINO TOMOKO;YOKOYAMA MINESUKE;WAKANA SHIGEHARU
分类号 C12N15/09;G01N33/00;A01K67/027;A61K38/00;A61K45/00;A61P3/10;C07K14/47;C12N5/10;C12N15/85;G01N33/15;G01N33/50 主分类号 C12N15/09
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