发明名称 |
PLASMIDE THERMOSENSIBLE ET MICRO-ORGANISME TRANSFORME AVEC CELUI-CI. |
摘要 |
A plasmid having a temperature-sensitive replication origin which has the following properties: a) capable of autonomous replication in Corynebacterium and being retained in Corynebacterium; and, b) when a cell containing said plasmid is cultured at 31 DEG to 37 DEG C., replication of the plasmid is inhibited and at the same time, the plasmid is removed from the cell body; and a method for performing homologous recombination in Corynebacterium using the plasmid. |
申请公布号 |
FR2667875(B1) |
申请公布日期 |
1995.05.05 |
申请号 |
FR19910012701 |
申请日期 |
1991.10.15 |
申请人 |
AJINOMOTO KK |
发明人 |
MASAKAZU SUGIMOTO;HIROYUKI KOJIMA;AKIKO TANAKA;HIROSHI MATSUI;KATSUAKI SATOH;TSUYOSHI NAKAMATSU |
分类号 |
C12N15/09;C12N1/21;C12N9/00;C12N9/12;C12N15/63;C12N15/77;C12P13/08;C12P13/22;C12R1/13 |
主分类号 |
C12N15/09 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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