| 摘要 |
<p>A new cross-linked Factor VIII polypeptide showing a coagulant activity of Factor VIII wherein the cross-linking is an inter-chain cross-linking. A cross-linked Factor VIIIa polypeptide showing a coagulant activity of Factor VIII wherein the cross-linking is an inter-chain cross-linking is stable at 4°C for an extended period. A new cross-linked Factor VIII polypeptide showing a coagulant activity of Factor VIII may be prepared by incubating the Factor VIII polypeptide with a cross-linking agent in a buffer having in the presence of a non-ionic surfactant whereafter lysine is added for quenching excess cross-linking agent optionally followed by isolation of the cross-linked Factor VIII polypeptide.</p> |
