| 摘要 |
<p>SUPPRESSIVE FACTOR OF ALLERGY A monoclonal hybridoma cell line was constructed for the production of suppressive factor of allergy (SFA). mRNA isolated from the monoclonal hybridoma cell line was identified by its ability to direct SFA synthesis in Xenopus laevis oocytes. The SFA produced by the monoclonal hybridoma cell line and the translational product of the mRNA in the oocytes was found effective in inhibiting IgE- specific receptor expression in dilutions greater than 1:1,000 of the culture supernatant. A sensitive bioassay for SFA was developed using cultures of thymocytes in which Lyt-2 cells were blocked with monoclonal antibodies, and subsequent IgE-specific receptor expression was determined in Lyt-1 cells.</p> |
