| 摘要 |
The extraction of Kallekrein from the pancreas and submandibularis gland of pigs is carried out by subjecting the fresh comminuted glands at about room temperature with an aqueous solution of a salt, such as sodium chloride, of a concentration of from 3 to 25%, preferably 8% of the salt, at a pH value of 5.5 to 9.5, preferably 7.5 to 8.5, in the presence of a water-miscible solvent such as methanol, and the mixture stirred for about 1 hour. The aqueous kallekrein-containing phase is separated from the solids and the kallekrein precipitated in the presence of kieselguhr and acetone. High yields and great reduction in extraction time are noted.
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