| 摘要 |
PROBLEM TO BE SOLVED: To obtain a new replicable DNA which contains a base sequence coding for aspartate-1-decarboxylase, is replicable in a microorganism such as corynebacteria and escherichia, and can be used for producing pantothenic acid by fermentation. SOLUTION: This is a new replicable DNA which consists of a nucleotide sequence consisting of panD gene coding for aspartate-1-decarboxylase, or a sequence corresponding to the sequence shown by the formula within the homology of the genetic code, sequences which hybridizes with sequences complementary to these sequences, is replicable in a microorganism such as corynebacteria and escherichia, can be a recombinant new DNA, and can be used for improved production of pantothenic acid by fermentation and so on. This DNA is obtained by extracting a chromosomal DNA from Corynebacterium glutamicum ATCC 13032, preparing a gene library using the obtained DNA by the conventional method, followed by screening the obtained library using a probe consisting of its partial sequence. |
