| 摘要 |
PROBLEM TO BE SOLVED: To obtain a general-purpose expression vector for a bacterium of the genus Rhodococcus, containing a mutant type regulatory factor having actions on constituent activation of a nitrilase gene promoter and capable of highly expressing the objective gene. SOLUTION: This expression vector for a bacterium of the genus Rhodococcus comprises a DNA region capable of coding a regulatory factor having actions on the activation of a nitrilase gene promoter, a nitrilase promoter gene DNA region undergoing the activation by the regulatory factor, a DNA region capable of proliferating in the bacterium of the genus Rhodococcus and a drug-resistant DNA region capable of functioning in the bacterium of the genus Rhodococcus. An exogenote is integrated into the expression vector for the bacterium of the genus Rhodococcus and made to coexist in the bacterial cell of the genus Rhodococcus to thereby enable the constituent expression of the exogenote. |
