| 摘要 |
1531651 Determination of methotrexate S E CHARM and H E BLAIR 19 Jan 1977 [20 Jan 1976] 02183/77 - Heading G1B Methotrexate in a body fluid sample containing at least 0À03 ng per ml of free methotrexate is quantitatively determined by a method comprising adding a known amount of dihydrofolate reductase and a known amount of I-125 labelled methotrexate to the sample, removing unbound methotrexate for example by adsorption on charcoal which has been treated with dextran gelatine, or haemoglobin and measuring the gamma ray count of the labelled bound methotrexate. |
