| 摘要 |
A coryneform expression and secretion system for homologous and heterologous genes consisting of the host, nucleotide sequences encoding a protein of interest, signals for expression and, optionally, targeting signals which direct membrane anchoring and for secretion and processing of the expressed protein. Regulatory signals may be utilized to control the rate and extent of expression and secretion. The system may further include compounds such as ionophores for altering the membrane transport of the host. The host itself may be mutated to alter transport, for example, by decreasing the mycolic acid content of Corynebacteria species. The preferred host is a Corynebacterium although other coryneforms deficient in extracellular protease production may also be used. C. glutamicum is used as a model organism for the secretion system. This Gram positive, non-pathogenic bacteria can efficiently utilize heterologous expression and secretion signals originating from a variety of both Gram negative and Gram positive bacteria to provide the basis for the overproduction and secretion of cloned gene products in a given Corynebacterium host, as demonstrated by the expression and secretion by C. glutamicum of a lipase encoded by a gene from S. hyicus and a thermonuclease encoded by a gene from S. aureus.
|
