发明名称 |
CELL-BASED FLUORESCENCE RESONANCE ENERGY TRANSFER (FRET) ASSAYS FOR CLOSTRIDIAL TOXINS |
摘要 |
The present invention provides a method of determining clostridial toxin activity by (a) contacting with a sample a cell containing a clostridial toxin substrate that includes a donor fluorophore; an acceptor having an absorbance spectrum overlapping the emission spectrum of the donor fluorophore; and a clostridial toxin recognition sequence containing a cleavage site that intervenes between the donor fluorophore and the acceptor, where resonance energy transfer is exhibited between the donor fluorophore and the acceptor under the appropriate conditions; (b) exciting the donor fluorophore; and (c) determining resonance energy transfer of the contacted cell relative to a control cell, where a difference in resonance energy transfer of the contacted cell as compared to the control cell is indicative of clostridial toxin activity. |
申请公布号 |
EP1543329(B1) |
申请公布日期 |
2016.08.03 |
申请号 |
EP20030774466 |
申请日期 |
2003.09.04 |
申请人 |
ALLERGAN, INC. |
发明人 |
FERNANDEZ-SALAS, ESTER;STEWARD, LANCE, E.;AOKI, KEI, ROGER |
分类号 |
G01N33/554;C07K14/035;C07K14/16;C07K14/33;C07K14/47;C12N5/10;C12Q1/10;C12Q1/37;G01N;G01N33/52;G01N33/533;G01N33/569;G01N33/68 |
主分类号 |
G01N33/554 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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