发明名称 |
IMMUNOASSAY FOR BREAST CANCER USING MONOCLONAL ANTIBODIES |
摘要 |
An immunoassay for diagnosing and monitoring human breast cancer. The assay employs a monoclonal antibody which recognizes a human mammary tumor virus derived from the T47D clone-10 breast cancer cell line (HMTV). The monoclonal antibody is produced by the hybridoma cell line deposited with the American Type Culture Collection under ATCC Accession No. HB 8630. When the immunoassay is performed on a tissue sample from a subject, the sample is contacted with monoclonal antibody ATCC Accession No. HB 8630 so as to form an antibody-antigen complex between the monoclonal antibody and any HMTV antigens which may be present in the sample. This antibody-antigen complex is then detected employing a second detectable antibody specific to the first monoclonal antibody. The presence or absence of the complex indicates the presence or absence of breast cancer in the subject. When the immunoassay is performed on a body fluid sample from a subject, the sample is first contacted with a detectable soluble antibody so as to form an antibody-antigen complex between the antibody and any HMTV antigens which may be present in the sample. The fluid sample is then contacted by a second antibody which is attached to a solid support. Either the first or second antibody is the monoclonal antibody produced by hybridoma cell line ATCC Accession No. HB 8630. The presence or absence of the complex indicates the presence or absence of human breast cancer in the subject. |
申请公布号 |
AU4649585(A) |
申请公布日期 |
1986.02.27 |
申请号 |
AU19850046495 |
申请日期 |
1985.08.21 |
申请人 |
TEL AVIV UNIVERSITY, TEVA PHARMACEUTICAL INDUSTRIES LIMITED |
发明人 |
IAFA KEYDAR |
分类号 |
C07K16/10;C07K16/30;C12P21/08 |
主分类号 |
C07K16/10 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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