摘要 |
<p>PURPOSE: To analyze creatine kinase MB contained in an aqueous biological fluid for catalytic activity by using an antibody mixture, a specific antibody contained in the mixture, hybridoma which secretes an antibody, and the mixture of the hybridoma. CONSTITUTION: A monoclonal antibody mixture is prepared by mixing a first monoclonal antibody which inhibits 55-65% of the activity of creatine kinase MM, does not inhibit the catalytic activity of creatine kinase MB, and can only be bound with creatine kinase MM, a second monoclonal antibody which inhibits 85-95% of the catalytic activity of creatine kinase MM and 35-45% of the catalytic activity of creatine kinase MB and can be evenly bound with the creatine kinase MM and creatine kinase MB, and a third monoclonal antibody which inhibits 85-95% of the catalytic activity of creatine kinase MM and 35-45% of catalytic activity of creatine kinase MB and is bound with creatine kinase MM more strongly than creatine kinase MB with each other.</p> |