ASSAY METHOD FOR DETERMINING PRODUCT R'S EFFECT ON INDUCTION OF IFN-GAMMA IN CULTURED CELLS

摘要

An assay method for determining the effect of Product R on expression of IFN-gamma in cultured cells. The method comprising the following step: 1) introducing Product R into cultured cells; 2) culturing the Product R-containing cultured cells for a period of time; 3) preparing total RNA from the cultured cells following the culturing; and 4) determining the amount of the INF-gamma mRNA in the total RNA preparation, which reflects the level of IFN-gamma expressing in cultured cells.