Selection of fungal resistant grape somatic embryos

摘要

The invention features a method of selecting for a grape somatic embryo or grape plant having resistance to a plant pathogen using a liquid culture medium. The method including the steps of (a) culturing a grape embryonic culture in a first liquid culture medium to grow a cellular suspension culture, the first liquid culture medium including a plant growth regulator and a filtrate from a plant pathogen culture; (b) recovering an embryogenic cell or embryogenic cell mass from the cellular suspension culture; and (c) culturing the embryogenic cell or embryogenic cell mass in a second liquid culture and in a third culture media to select a grape somatic embryo or a grape plant having resistance to a plant pathogen.