Use of sulfonic derivatives of acylated polyethyleneimine bonded phase silica products

摘要

Sulfonic derivatives of N-acylated covalently bound, non-crosslinked polyethyleneimine bonded phase silicas of the formula: +TR wherein is the backbone of a silica gel or glass, n is an integer such that the polyethyleneiminopropyl silane group has an average molecular weight of from about 400 to about 1800; q is an integer of zero or one; R is selected from the group consisting of hydrogen, -CH2)xH or -CH2)mCOOH where x is an integer of 1 or 2 and m is an integer of zero or 1; when R is hydrogen or -CH2)xH then R1 is -CH2)p COOH where p is an integer of zero or 1, and when R is -CH2)mCOOH then R1 is selected from the group consisting of hydrogen or -CH2)yH where y is a integer of zero or 1, are especially useful as solid phases for the purification and separation of proteins having isoelectric points of below about 5.