METHOD FOR FORMING SAMPLE OF STRATUM CORNEUM

摘要

PURPOSE:To form a sharp sample easy to observe and having good preservability, by adhering a tape wherein a lining stratum corneum is adhered to an adhesive substance soluble in an org. solvent to a transparent plate coated with a fixing agent and immersing the whole in the org. solvent to peel off the tape. CONSTITUTION:A tape coated with a viscous substance composed of a resin or high-molecular compound soluble in org. solvent is closely contacted with a cortical layer and peeled off to sample a part of a stratum corneum on the tape. This tape is adhered to a transparent plate preliminarily coated with a fixing agent and the whole is immersed in the org. solvent for several hr to dissolve and swell the viscous substance and the tape is subsequently peeled off. The fixing agent applied to the transparent plate is set to a substance not modifying the cells of the stratum corneum and hardly soluble in the org. solvent and the tape is a narrow one and improved in the permeability of the solvent. This sample is stained and sealed in balsam to make it possible to obtain a sharp sample easy to observe and improved in preservability.