摘要 |
This invention pertains to a process for accurately and specifically determining and/or dosing in a fluid or semi-fluid medium at least one biological, organic or drug-soluble substance, even if it is present only in an extremely minute quantity, by using an appropriate agglutination reaction. Using fluorescent structures, hereafter referred to as spheres, capable of binding a suitable antiligand, this process is characterized in that the sample to be analyzed is brought into contact with one homogenous population of spheres for each ligand, if up to two ligands are to be determined, and with less than one population of spheres for each ligand, if three ligands and more are to be determined. It is further characterized in that the number of non-agglutinated spheres, the number of agglutinated spheres, the number of sphere aggregates, and, for each aggregate, the number of spheres it comprises are determined and/or counted simultaneously by a measuring means with a single parameter for measuring fluorescence by ligand, said process making it possible to determine and/or dose several ligands simultaneously by contact only. Application to determining and/or dosing biological substances. |