发明名称 |
Augmenter of liver regeneration (ALR) |
摘要 |
A full-length cDNA clone has been isolated encoding a purified augmenter of liver regeneration (ALR) polypeptide prepared from the cytosol of livers from weanling rats. The 1.2 kb cDNA includes a 299 bp 5'-untranslated region, a 375 bp coding region and a 551 bp 3'-untranslated region. The cDNA encodes a protein consisting of 125 amino acids. The molecular weight of ALR calculated from the cDNA was 15,081 which is consistent with the size estimated by SDS-PAGE under reducing conditions. The molecular weight of the purified native ALR estimated by SDS-PAGE under non-reducing conditions is about 30,000, apparently with a homodimer structure. The recombinant ALR produced by expression of the cDNA in cultured cells was tested in vivo in the canine Eck's fistula model and found to have a potency equivalent to the purified native ALR. The most obvious immediate clinical use for the augmenter of liver regeneration is in the treatment of hepatic failure.
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申请公布号 |
US5480797(A) |
申请公布日期 |
1996.01.02 |
申请号 |
US19940197496 |
申请日期 |
1994.02.16 |
申请人 |
TOYOBO CO., LTD.;UNIVERSITY OF PITTSBURGH |
发明人 |
FRANCAVILLA, ANTONIO T.;HAGIYA, MICHIO;STARZL, THOMAS E. |
分类号 |
A61K38/00;C07K14/47;C12N15/12;(IPC1-7):C12N1/21;C12N5/10;C12N15/63 |
主分类号 |
A61K38/00 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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