| 摘要 |
<p>Isolated CagB gene of H. pylori consisting of nucleotides 193-1158 in the sequence according to Seq. ID. No. 1 and isolated cagC gene of H. pylori with sequence consisting of nucleotides 1170-3830 in the sequence according to Seq. ID. No. 3 are provided, as well as H. pylori specific nucleic acid probes and purified CagB and CagC proteins. CagB and CagC are associated with peptic ulceration and other clinical syndromes in humans infected with H. pylori expressing these antigens. Provided is a method of detecting presence of H. pylori strains expressing CagB or CagC antigens in subject, comprising contacting antibody-containing sample from subject with detectable amounts of CagB or CagC or specific antigenic fragments, detecting binding reaction of CagB or CagC or antigenic fragments and antibody produced by subject. Mutant strains of H. pylori lacking functional CagB or CagC are virulence attenuated due to reduced production of proinflammatory cytokine IL-8.</p> |
