摘要 |
FIELD: toxicology, in particular determination of water flea sensibility to toxic effect of water-soluble chemicals. ^ SUBSTANCE: claimed method includes detection of water flea death time (min) caused by water-soluble chemicals, wherein concentration (C, mol/l) of chemical under consideration fluctuates according to logarithmic scale with interval of 0.1. Plot of Y versus X is made, wherein Y-axis represents average death time with scale of 1 point = 1 min; X-axis represents reverse concentration (1/C) of chemical under consideration; and scale is proportional to log increasing by 0.1. Water flea sensibility to toxic effect (tgalpha) is calculated according to equation: tgalpha = TL(min):1/KL = TL(min)xKl (I), wherein alpha is inclination of straight line to X-axis; TL(min) is death time (min) being determined according to point of hypothetical crosspoint of straight line with Y-axis; KL is lethality constant (mol/l) defined as chemical concentration wherein water flea death time is equal to 2TL(min). ^ EFFECT: Method allowing evaluation of toxic effect evolution dynamics and comparison of toxic effect of water-soluble chemicals in equal concentration ranges. ^ 2 tbl, 1 ex, 1 dwg
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