PRODUCTION OF DIFFERENTIATION-INDUCING SUBSTANCE DERIVED FROM HUMAN

摘要

PURPOSE:To produce bioactive substance having cell differentiating and inducing (differentiate tumor cell to cell having normal function) activity by culturing meacrophage-like cell derived from human infected with mycoplasma. CONSTITUTION:A cell derived from human contaminated with mycoplasma is cultured, or cell and crushed material of mycoplasma performed of inactivation treatment is added to a culturing system of cell derived from human to produce cell differentiating and inducing substance in said culturing system. In said process, differentiating and inducing agent such as pholbol esters, diterpene-based compound represented by mezerein or teleocidin is preferably added to the culturing system. Resultant differentiating and inducing substance has an amino acid sequence expressed by the formula (X is amino acid impossible or difficult to identify by Edman decomposing method) in amino chain end and has about 51000 molecular weight (be gel filtration technique).