PEROXIDASE COLLOIDAL GOLD OXIDASE BIOSENSORS FOR MEDIATORLESS GLUCOSE DETERMINATION

摘要

Horseradish peroxidase (HRP) immobilized on colloidal gold and then deposite d on an electrode surface can be reduced at a convenient rate at low voltage (Ag/AgCl) without an electron transfer mediator. Bioelectrodes combining both a colloidal gold-adsorbed oxidase and colloidal gold-adsorbed HRP located on an electrode surface are efficient biodetectors, particularly for the measurement of low glucose levels in samples when glucose oxidase is employed as the sensing enzyme. Th e biodetectors may be employed for mediatorless detection if a wide variety of analytes depending on the oxidase employed.