| 发明名称 | Nucleic acid production and sequence analysis | ||
| 摘要 | A method for producing a nucleic acid molecule from a template nucleic acid sequence and a linking unit attached to a primer, which method comprises a step of contacting the template nucleic acid sequence with a nucleic acid polymerase under conditions which allow the nucleic acid polymerase to produce the nucleic acid molecule from the primer based on the template nucleic acid sequence, wherein the linking unit is attached to a target site in the template nucleic acid sequence with a covalent linkage. | ||
| 申请公布号 | US9347093(B2) | 申请公布日期 | 2016.05.24 |
| 申请号 | US201213679538 | 申请日期 | 2012.11.16 |
| 申请人 | Vilnius University | 发明人 | Klimasauskas Saulius;Stasevskij Zdislav |
| 分类号 | C12Q1/68;C12P19/34 | 主分类号 | C12Q1/68 |
| 代理机构 | Thompson Hine LLP | 代理人 | Thompson Hine LLP |
| 主权项 | 1. A method for producing a nucleic acid molecule from a template nucleic acid sequence comprising a linking unit attached to a primer, which method comprises a step of contacting the template nucleic acid sequence with a nucleic acid polymerase under conditions which allow the nucleic acid polymerase to produce the nucleic acid molecule from the primer based on the template nucleic acid sequence, wherein the linking unit is attached with a covalent linkage to a target site within the template nucleic acid sequence and not to a terminal nucleotide of the template nucleic acid sequence such that priming of nucleic acid synthesis occurs at or within less than 5 bases of the target site. | ||
| 地址 | Vilnius LT | ||