Method for producing corneal endothelial cell

摘要

The invention provides a method of efficiently producing corneal endothelial cells, particularly from corneal stroma or iPS cell-derived neural crest stem cells, a method of producing corneal endothelial cells stably in a large amount by inducing more efficient differentiation of stem cells into corneal endothelial cells, and a medicament containing corneal endothelial cells. The method of inducing differentiation of stem cells into corneal endothelial cells includes a step of culturing the stem cells in a differentiation induction medium containing a GSK3 inhibitor (preferably a GSK3β inhibitor) and retinoic acid, with the differentiation induction medium preferably further containing one or more of TGFb2, insulin, a ROCK inhibitor, and the like.

主权项

1. A method of producing a corneal endothelial cell from a human iPS cell-derived neural crest stem cell, comprising a step of cultivating the stem cell in a differentiation induction medium, wherein the differentiation induction medium is a serum-containing medium comprising a glycogen synthase kinase 3 (GSK3) inhibitor, retinoic acid, transforming growth factor (TGFb2), insulin, and a Rho-associated protein kinase (ROCK) inhibitor.