| 发明名称 | Enhanced expression method | ||
| 摘要 | The present invention concerns a method of producing a desired heterologous gene product wherein said heterologous gene product is expressed from a strong promoter, said method comprising expressing said gene using a mutant mRNA leader which comprises one or more mutations which enhance transcription of the gene. The invention also provides a mutant Pm mRNA leader sequence, and a vector and a library comprising the leader sequence. Methods of obtaining an mRNA mutant leader and identifying an mRNA mutant leader are encompassed, along with a vector for selection or identification of an mRNA leader mutant and a use thereof for screening. | ||
| 申请公布号 | US9388421(B2) | 申请公布日期 | 2016.07.12 |
| 申请号 | US200712376312 | 申请日期 | 2007.08.03 |
| 申请人 | VECTRON BIOSOLUTIONS AS | 发明人 | Valla Svein;Lale Rahmi;Berg Laila;Bakke Ingrid |
| 分类号 | C12N15/70;C07H21/04 | 主分类号 | C12N15/70 |
| 代理机构 | Porter, Wright, Morris & Arthur LLP | 代理人 | Porter, Wright, Morris & Arthur LLP |
| 主权项 | 1. A method of producing a desired heterologous gene product in a prokaryotic expression system, said method comprising expressing a gene encoding said heterologous gene product from a promoter at a level of at least 1% of the total cellular protein in a prokaryotic host cell using a mutant Pm mRNA leader having a sequence selected from any one of SEQ ID NOs 2-12 and 14-37. | ||
| 地址 | Trondheim NO | ||