Alternative export pathways for vector expressed RNA interference

摘要

The present invention is directed to nucleic acid molecules containing a loop sequence designed to circumvent exportin-5 mediated export, and methods using these novel molecules.

主权项

1. A method of reducing the expression of a gene in a cell, comprising contacting the cell with an isolated nucleic acid comprising:
a first portion, wherein the first portion is no more than 30 nucleotides in length; a second portion, wherein the second portion has a sequence that is complementary to the first portion; and a loop portion consisting of 12 to 50 nucleotides which comprise between 12 and 32 nucleotides of SEQ ID NO:1 and is designed to circumvent exportin-5 mediated export; wherein the first portion and the second portion are operably linked by means of the loop portion to form a hairpin structure comprising a duplex structure and a loop structure.