Chimeric DNA polymerases

摘要

The present invention provides, among other things, chimeric DNA polymerases containing heterologous domains having sequences derived from at least two DNA polymerases that have at least one distinct functional characteristics (e.g., elongation rate, processivity, error rate or fidelity, salt tolerance or resistance) and methods of making and using the same. In some embodiments, the present invention can combine desired functional characteristics (e.g., high processivity; high elongation rate; thermostability; resistance to salt, PCR additives (e.g., PCR enhancers) and other impurities; and high fidelity) of different DNA polymerases in a chimeric polymerase.

主权项

1. A DNA polymerase whose amino acid sequence contains, in order:
an N-terminal domain including a sequence that shows at least 90% identity with that sequence between residues 26 and 105 of SEQ ID NO: 16; an exonuclease domain including a sequence that shows at least 90% identity with that sequence between residues 156 and 301 of SEQ ID NO: 16; a palm and finger domain including a sequence that shows at least 90% identity with that sequence between residues 394 and 563 of SEQ ID NO: 16; and a thumb domain including a sequence that shows at least 90% identity with that sequence between residues 612 and 749 of SEQ ID NO: 16.