DNA PROMOTER FRAGMENTS FROM WHEAT

摘要

The preparation and use of nucleic acid promoter fragments homologous to the Em gene of wheat to bring the expression of selected genes in plants under external control are described. The Em promoter fragment is responsive to abscisic acid (ABA) and other compounds possessing ABA-like activity. Through transformation of protoplasts and plant cells with recombinant DNA constructs incorporating such promoter fragments, operably linked selected genes are expressed in response to ABA and compounds possessing ABA-like activity. The application of such promoter fragments and constructs to transient assay systems to predict the likelihood of stable transformation in plants is disclosed.