Compositions, methods and kits for enhancing PCR specificity

摘要

The present disclosure provides novel primers and method for the detection of specific nucleic acid sequences. The primers and methods provided herein are useful in a wide variety of molecular biology applications and are particularly useful in allele-specific PCR.

主权项

1. A composition comprising
a first oligonucleotide primer comprising a 5′ Sequence-Targeted Amplification Restrictive (STAR) tag sequence portion and a target nucleic acid hybridization sequence portion; and a second oligonucleotide primer comprising a target nucleic acid hybridization sequence portion, wherein the STAR tag sequence is the same as all or a portion of the target nucleic acid hybridization sequence of the second oligonucleotide primer and the STAR tag sequence is the same as a sequence in the target nucleic acid 5′ of the first oligonucleotide target nucleic acid hybridization sequence hybridization site.