Maize ethylene signaling genes and modulation of same for improved stress tolerance in plants

摘要

The invention provides isolated maize EIN3, EBF1 and EIN5 nucleic acids and encoded proteins which are associated with ethylene signaling in plants. The present invention provides methods and compositions relating to altering ethylene sensitivity in plants. The invention further provides recombinant expression cassettes, host cells, transgenic plants and antibody compositions.

主权项

1. A recombinant expression cassette comprising a polynucleotide selected from the group consisting of:
(a) a polynucleotide encoding a polypeptide which has at least 95% sequence identity, as determined by the GAP algorithm under default parameters, to a polypeptide of SEQ ID NO: 4; (b) a polynucleotide encoding the polypeptide of SEQ ID NO: 4; (c) a polynucleotide of SEQ ID NO: 3; (d) a polynucleotide which has at least 95% sequence identity, as determined by the GAP algorithm under default parameters, to the full length of SEQ ID NO: 3; (e) a polynucleotide which has at least 95% sequence identity, as determined by the GAP algorithm under default parameters, to the full length of the coding sequence of SEQ ID NO: 3; and (f) a polynucleotide which is fully complementary to the polynucleotide of (a), (b), (c), (d), or (e), wherein said polynucleotide is operably linked, in sense or anti-sense orientation, to a heterologous promoter.