NOUVEAU PROCEDE DE RECUPERATION ET DE DOSAGE DE MICROQUANTITES D'ACIDE DESOXYRIBONUCLEIQUE DANS UN LIQUIDE BIOLOGIQUE ACELLULAIRE

摘要

The method disclosed is intended for the recovery and dosing of microquantities of DNA and comprises a first step of elimination of the proteins which are present, a second step of recovery of the DNA, which step comprises the precipitation of DNA by addition of a coprecipitation agent and a precipitation agent, a third step of marking by ''Nick Translation'' of the DNA to be recovered, and a fourth step of dosing. Application to the recovery and dosing of microquantities of DNA in an acellular biological liquid.