| 摘要 |
Bacteria in a fluid sample are conc., immobilised and stained by (1) electrostatically-adsorbing bacteria to a specific portion of a negatively-charged filter; (2) staining the adsorbed bacteria with a dye effective at basic pH; and (3) diffusing out free dye to a different region of the filter, leaving stained bacteria in position for comparative quantitative analysis. Also new is an appts. for this process. - Pref. the sample is acidified with HCl, HNO3 or H2SO4 of pH 1-3, and the dye is Safranin O or basic fuschin, effective at pH 8-12, dissolved at 0.001-0.1% in a pH 8-12 buffer (K borate-K2CO3-KOH).
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