| 摘要 |
There is disclosed a process for screening an agent to determine whether it increases the frequency of genome rearrangement in living matter. In the first step of this process, there is provided viable mammalian cells which comprise repeated genetic elements in their haploid genome. These repeated genetic elements are selected from the group consisting of functional and non-functional genetic elements; and these elements are sufficiently homologous so that, under ambient conditions, they recombine with each other and give rise to an identifiable genome rearrangement. In the second step of this process, the viable mammalian cells are exposed to the agent to be tested. Thereafter, they are incubated in growth medium which, after the exposed cells grow in it, facilitates the identification of those cells which have undergone said genome rearrangement. In the last step of the process, the extent to which the exposed mammalian cells have undergone genome rearrangement is determined. Also disclosed is the viable mammalian celline used in said process, the plasmid used to construct said celline, and a process for constructing said celline.
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