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1、一种生产具有至少一种IL-3样生物学特性的灵长类多肽的方法，该方法包括培养用重组DNA载体转化的细胞，该重组DNA载体可编码包括如下所示成熟肽序列的多肽，a) 10 20 30 49CTCGAGCTAC GTCAACGAAA AATAAAATCC AAAC ATG AGC TGC CTG CCC GTC CTG CTC MET Scr Cys Leu Pro Val Leu Leu 64 79 94 109CTG CTC CAA CTC CTG GTC AGC CCC CGA CTC CAA GCT CCC ATG ACC CAG ACA ACGLey Leu Gln Leu Leu Val Ser Pro Gly Leu Gln Ala Pro MET Thr Gln Thr Thr124 139 154TCC TTG AAG ACA AGC TGG GTT AAC TGT TCT AAC ATG ATC GAT GAA ATT ATA ACASer Leu Lys Thr Ser Trp Val Asn Cys Ser Asn MET Ile Asp Glu Ile Ile Thr169 184 199 214CAC TTA AAG CAG CCA CCT TTG CCC TTG CTG GAC TTC AAC AAC CTC AAT GGG GAAHis Leu Lys Gln Pro Pro Leu Pro Leu Leu Asp Phe Asn Asn Leu Asn Gly Glu229 244 259 274GAC CCA GAC ATT CTG ATG GAA AAT AAC CTT CGA AGG CCA AAC CTG GAG GCA TTCAsp Gln Asp Ile Leu MET Glu Asn Asn Leu Arg Arg Pro Asn Leu Glu Ala Phe289 304 319AAC AAG GCT GTC AAG AGT TTA CAG AAT GCA TCA GCA ATC GAG AGC ATT CTT AAGAsn Lys Ala Val Lly Ser Leu Gln Asn Ala Ser Ala Ile Glu Ser Ile Leu Lvs334 349 364 379AAT CTC CCC CCA TGC CTG CCC ATG GCC ACA GCC GCA CCC ACG CGA CAT CCA ATCAsn Leu Pro Pro Cys Leu Pro MET Ala Thr Ala Ala Pro Thr Arg His Pro Ile394 409 424CGT ATC AAG GAC CGT GAC TGG AAT GAA TTC CGC ACG AAA CTG AAG TTC TAT CTGArg Ile Lys Asp Gly Asp Trp Asn Glu Phe Arg Arg Lys Leu Lys The Tyr Leu439 454 469 484AAA ACC CTT GAG AAT GAG CAA GCT CAA CAG ATG ACT TTG AGC CTT GAG ATC TCTLys Thr Leu Glu Asn Glu Gln Ala Gln Gln MET The Leu Ser Leu Glu Ile Ser500 510 520 530 540 550 560TGAGTCCAAC GTCCAGCTCT CTCTCTGGGC CGTCTCACCG CAGAGCCTCA GGACATCAAA AACAGCAGAA570 580 590 600 610 620 630CTTCTGAAAC CTCTGGGTCG TCTCTCACAC AGTCCACGAC CACAAGCATT TCACCTTTTC CTGOGGCATC640 650 660 670 680 690 700AGATGAATTG TTAATTATCT AATTTCTGAA ATGTGCAGCT CCCATTTGGC CTTGTGTGGT TGTGTTCTCA710 720 730 740 750 760 770TTTTTATCCC ATTGAGACTA TTTATGTATG TCTCTATTTA TTTATTTATT TATTTATTGC CTTCTGGAGC780 790 800 810 820 830 840GTGAAGTGTA TTTATTTCAG CAGAGGAGCC ATCTCATGCT GCTTCTGCAA AAAATCCAAG AGTGGGGTGG850 860GGAGCATGTT CATTTGTACC TCGAGb)<img file="871048159_IMG1.GIF" wi="800" he="774" />429384 399 A 414 A AAAT GAA TTC CGG ACG AAA CTG ACG TTC TAT CTG AAA ACC CTT GAG AAT GCG CAGAsn Glu Phe Arg Arg Lys Leu Thr Phe Tyr Leu Lys Thr Leu Glu Asn Ala Gln130 Lys444 459 485 T T A C G 475 TC 495GCT CAA CAG ACG ACT TTG AGC CTC GCG ATC TTT T-AGTCCAACG TCCAGCTCGT TCTCTGGGCCAla Gln Gln Thr Thr Leu Ser Ldu Ala Ile Phe MET 147 Glu Ser 505 515 555C G A A 525 535 545 G 565TTCTCACCAC AGCGCCTCCG GACATCAAAA ACAGCAGAAC TTCTGAAACC TCTCGGTCAT CTCTCACACAG 575 585 595 605 615 625 635TTCCAGGACC AGAAGCATTT CACCTTTTCC TCCCGCATCA GATGAATTGT TAATTATCTA ATTTCTGAAA 645 655 T 665TGTGCAGCTC CCATTTGGCC TTGTGCGGTT GTGTTCTCA所述的生物学特性选自下列一组：a)维持来自红细胞样、淋巴样和髓细胞样谱系的灵长类祖先细胞的生长和分化的能力；b)在标准人骨髓测定中刺激粒性白细胞集落和红细胞样释放物的能力；c)维持灵长类多能性前体细胞生长的能力；d)在慢性骨髓性白血病(CML)测定中以10-100微微克分子的浓度刺激CML细胞的能力；e)刺激组胺在脾和骨髓细胞中的合成增加的能力；和f)刺激KG-1细胞集落形成的能力。 |
