发明名称 |
Method for cultivation of hepatocytes |
摘要 |
<p>The present invention provides a method for cultivation of hepatocytes efficiently by using a hepatocyte growth promoting factor derived from 3T3 cells, which comprises culturing hepatocytes isolated from the liver of a matured mammal in a medium containing pleiotrophin, fetal bovine serum, ascorbic acid or analogues thereof and nicotinamide or an analogue thereof, thereby forming primary colonies of the hepatocytes; and a method for subculture of the primary hepatocytes in the same medium. <IMAGE></p> |
申请公布号 |
EP0882787(A1) |
申请公布日期 |
1998.12.09 |
申请号 |
EP19980304469 |
申请日期 |
1998.06.05 |
申请人 |
JAPAN SCIENCE AND TECHNOLOGY CORPORATION |
发明人 |
SATO, HAJIME;YOSHISATO, KATSUTOSHI |
分类号 |
C12N1/38;C07K14/475;C12N5/071;(IPC1-7):C12N5/06 |
主分类号 |
C12N1/38 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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