ANTI-ANGIOGENESIS PLASMIDS AND DELIVERY SYSTEMS, AND METHODSOF MAKING AND USING THE SAME

摘要

The present invention relates to gene delivery and gene therapy, and provide s novel nucleic acid constructs for expression of anti-angiogenic agents in a mammal, formualtions for delivery that incorporate a nucleic acid construct for expression, and methods for preparing and using such constructs and formulations. In particular, this invention relates to plasmid constructs fo r delivery of therapeutic anti-angiogenic encoding nucleic acids to cells in order to modulate tumor activity, methods of using those constructs (includi ng combination therapy with other agents, such as cytokines, preferably IL-12), as well as methods for preparing such constructs.