| 摘要 |
Disclosed herein is a method for the detection of a target substance by a colloidal gold immunoassay, which comprises dissolving in an immunoreaction system a metal salt selected from the group consisting of sodium, potassium and lithium fluorides, sodium, potassium, lithium and magnesium iodides, sodium, potassium, lithium and magnesium bromides, lithium and magnesium chlorides, sodium, potassium, lithium and magnesium nitrates, sodium, potassium, lithium and magnesium sulfates, sodium, potassium, lithium and magnesium formates, sodium, potassium, lithium and magnesium acetates, and mixtures of at least two of these metal salts, whereby the metal salt is allowed to exist in a reaction mixture. According to this assay method, the development of a color by the colloidal gold becomes very vivid owing to a function of the metal salt. When observing with the naked eye especially a specimen of a concentration around a detection limit, a difference between negativity and positivity becomes more distinct, thereby making it possible to make a judgment with ease. |
