NOVEL COMPOUND CHAETOATROSIN AN INHIBITING CHITIN SYNTHEASE 2 AND ANTIMYCOTIC AGENT COMPOSITION CONTAINING THE SAME

摘要

PURPOSE: A novel compound chaetoatrosin A inhibiting chitin synthease 2 is provided which has excellent and selective inhibition activities. Also, an antimycotic agent composition containing the same is provided which has excellent antibacterial activity against true fungi. CONSTITUTION: A novel compound chaetoatrosin A inhibiting chitin synthease 2 represented by the formula (1) is prepared by a Chaetomium atrobrunneum F449 strain(KCTC 0612 BP). A purification method of chaetoatrosin A from the culture of Chaetomium atrobrunneum F449 comprises the following steps: (i)obtaining crude extract by adding ethyl acetate to Chaetomium atrobrunneum F449 culture and by extracting; (ii)obtaining an active fraction by separating the crude extract with silica gel column chromatography which uses mixed liquid of chloroform-methanol as eluate; (ii)obtaining an active fraction by separating the active fraction in step(ii) with reverse phase silica gel column chromatography which uses mixed liquid of methanol-water as eluate; (iv)obtaining partially purified materials by separating the active fractions in step(iii) with sephadex LH-20 column chromatography which uses methanol as eluate; (v)separating pure chaetoatrosin A by separating and purifying the partially purified materials with preparative TLC.