Reporter genes for magnetic resonance imaging and methods of use thereof

摘要

The invention provides a fusion ferritin protein wherein a ferritin heavy chain polypeptide is fused to a peptide, wherein the peptide is fused to the C-terminal end of the ferritin heavy chain; and the peptide includes at least a portion of a Mms6 protein sequence and at least one heterologous amino acid at its N-terminal end. The invention further provides methods of use of the ferritin fusion protein for Magnetic Resonance Imaging.

主权项

1. A nucleic acid sequence encoding a recombinant ferritin heavy chain fusion protein, comprising a nucleic acid sequence encoding a ferritin heavy chain polypeptide fused to a peptide, wherein said peptide is fused to the C-terminal end of said ferritin heavy chain; said peptide comprising at least a portion of a Mms6 protein sequence and at least one heterologous amino acid at its N-terminal end, wherein said portion of the Mms6 protein sequence is able to bind magnetite, and wherein said portion of the Mms6 protein comprises the amino acid sequence of SEQ ID NO: 2.