发明名称 |
Immunogenic POTE peptides and methods of use |
摘要 |
POTE has recently been identified as a tumor antigen expressed in a variety of human cancers, including colon, ovarian, breast, prostate, lung and pancreatic cancer. Described herein are immunogenic POTE polypeptides, including modified POTE polypeptides, that bind MHC class I molecules. The immunogenic POTE polypeptides are capable of inducing an immune response against POTE-expressing tumor cells. Thus, provided herein is a method of eliciting an immune response in a subject, such as a subject having a type of cancer that expresses POTE. |
申请公布号 |
US9394352(B2) |
申请公布日期 |
2016.07.19 |
申请号 |
US201414496194 |
申请日期 |
2014.09.25 |
申请人 |
The United States of America, as represented by the Secretary, Department of Health and Human Services |
发明人 |
Berzofsky Jay A.;Huang Yi-Hsiang;Terabe Masaki;Pastan Ira H. |
分类号 |
C07K7/06;C07K14/47;A61K39/00;A61K48/00 |
主分类号 |
C07K7/06 |
代理机构 |
Klarquist Sparkman, LLP |
代理人 |
Klarquist Sparkman, LLP |
主权项 |
1. An isolated nucleic acid molecule encoding an immunogenic polypeptide, wherein the polypeptide comprises no more than 10 consecutive amino acids of the amino acid sequence of human POTE set forth as:(SEQ ID NO: 20)MVAEVCSMPTASTVKKPFDLRSKMGKWCHHRFPCCRGSGKSNMGTSGDHDDSFMKMLRSKMGKCCRHCFPCCRGSGTSNVGTSGDHENSFMKMLRSKMGKWCCHCFPCCRGSGKSNVGAWGDYDHSAFMEPRYHIRREDLDKLHRAAWWGKVPRKDLIVMLRDTDMNKRDKEKRTALHLASANGNSEVVQLLLDRRCQLNVLDNKKRTALIKAIQCQEDECVLMLLEHGADRNIPDEYGNTALHYAIYNEDX1LX2AKAX3LX4YGADIESKNKCGLTPLLLGVHEQKQQVVKFLIKKKANLNVLDRYGRTALILAVCCGSASIVNX5LX6EQNX7DVSSQDLSGQTAREYAVSSHHHVICELLSDYKEKQMLKISSENSNPEQDLKLTSEEESQRLKVSENSQPEKMSQEPEINKDCDREVEEEIKKHGSNPVGLPENLTNGASAGNGDDGLIPQRRSRKPENQQFPDTENEEYHSDEQNDTRKQLSEEQNTGISQDEILTNKQKQIEVAEQKMNSELSLSHKKEEDLLRENSVLQEEIAMLRLELDETKHQNQLRENX8X9X10EEIX11SVKEKTDKLLRAMQLNEEALTKTNI, wherein X1 is K or Y; X2 is M, A or F; X3 is L or A; X4 is L or V; X5 is L or Y; X6 is L, A or F; X7 is V, or A; X8 is K or Y; X9 is I or L; X10 is L or F; and X11 is E or A; and wherein the polypeptide comprises one of (a) amino acids 323-331; (b) amino acids 252-260; or (c) amino acids 553-561 of SEQ ID NO: 20, wherein the polypeptide does not comprise the amino acid sequence KLMAKALLL (SEQ ID NO: 3). |
地址 |
Washington DC US |