DETECTION OF CANCER BY ELEVATED LEVELS OF BCL-2

摘要

The present invention relates to a method for the diagnosis, prognosis, and monitoring of cancer, such as early or late stage ovarian cancer, in a subject by detecting Bcl-2 in a biological sample from the subject, preferably a urine or blood sample. Bcl-2 may be measured using an agent that detects or binds to Bcl-2 protein or an agent that detects or binds to encoding nucleic acids, such as antibodies specifically reactive with Bcl-2 protein or a portion thereof. The invention further relates to kits for carrying out the methods of the invention. The invention further relates to a device for the rapid detection of Bcl-2 in a bodily fluid and methods for rapidly measuring Bcl-2 in a bodily fluid.

主权项

1. A method for determining a Bcl-2 protein level in a urine sample from a subject, the method comprising:
(a) obtaining the urine sample from the subject; (b) contacting the urine sample with a device comprising:
i) an application zone for receiving a urine sample;ii) a labeling zone containing a binding agent that binds to Bcl-2 protein in the sample; andii) a detection zone where Bcl-2 protein-bound binding agent is retained to give a signal that indicates that Bcl-2 protein level in the urine sample is lower than a threshold level and to give a different signal that indicates that Bcl-2 protein level in the urine sample is at or above the threshold level;and (c) detecting a signal from the detection zone of the device to determine the Bcl-2 protein level in the urine sample.