DNA-Sequenz, die ein Polypeptid mit Nitrilhydratase-Aktivität codiert, und Verfahren zur Herstellung von Amiden aus Nitrilen mit einer Transformante, die die DNA-Sequenz enthält

摘要

A DNA which encodes polypeptide carrying two specific amino acid sequences and having nitrile hydratase activity; a method for producing nitrile hydratase by incubating a transformant made by the transformation with a recombinant DNA prepared by integrating the foregoing DNA into a vector in a medium and harvesting nitrile hydratase accumulated in the medium; and a method for producing amides by incubating the foregoing transformant and then converting nitriles into corresponding amides by the action of the obtained nitrile hydratase or by making a culture solution, isolates, treated cells or their immobilized products act upon nitriles to produce corresponding amides.