Gardnerella vaginalis assay

摘要

The present invention relates to nucleic acid amplification assays for the detection of nucleic acid sequences of Gardnerella vaginalis. The present invention provides oligonucleotides that are complementary or that anneal to nucleic acid sequences of the vly gene of GV. The present invention also provides internal amplification controls (IACs) that can be used in nucleic acid amplification reactions.

主权项

1. A method for detecting a Gardnerella vaginalis (GV) target sequence if present in a sample comprising:
(a) providing at least one oligonucleotide primer that will anneal to at least some portion of a target region of the vly gene of GV selected from the group consisting of SEQ ID NO. 20 and SEQ ID NO. 21; (b) combining the at least one oligonucleotide primer with a biological sample; (c) subjecting the sample to conditions to cause amplification of a portion of the target sequence, if present, in the sample; and (d) determining the presence or absence of the amplified target sequence.