| 摘要 |
A two-hybrid system that can detect homo- and heterodimeric protein interactions in E. coli and other cells. This system is useful for the same applications as a yeast two-hybrid system, i.e. interaction cloning, mapping protein interaction domains, analysing protein interactions, detecting protein interactions and detecting modulators thereof. The invention concerns a prokaryotic host cell comprising: a) a fusion protein having (i) a first DNA-binding domain and (ii) a first interacting domain; b) a fusion protein having (i) a second DNA-binding domain and (ii) a second interacting domain capable of binding to the first interacting domain; and c) a nucleic acid molecule having a reporter gene operatively linked to (i) a promoter, (ii) a first operator site capable of binding to the first DNA-binding domain, located upstream of the promoter, and (iii) a second operator site capable of binding the second DNA-binding domain, located downstream of the promoter of the reporter gene; wherein binding of the first interacting domain to the second interacting domain is signaled by altered expression of the reporter gene.
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