发明名称 |
Method for isolating polyphenols from olive mill water |
摘要 |
The present invention relate to a highly efficient and novel method, using clean technologies, for obtaining a natural bioactive concentrate that is rich on polyphenols from olive mill water (OMW). The clean technologies integrate centrifugation, a drowning-out crystallization-based separation process, and vacuum evaporation. The method provides a highly-concentrated polyphenol isolate (up to 99% (mass fraction)) from other components presents in OMW, with up to half of the polyphenol content being hydroxytyrosol. The isolated polyphenols exhibit anti-oxidant, anti-microbial, anti-inflammatory, and anti-carcinogenic activities; they can be prepared as solid particles, as an aqueous solution, in an emulsion, or as lipidic-based nanoparticles. The isolated polyphenols can be used in the food industry, cosmetic industry, or pharmaceutical industry. |
申请公布号 |
US9420820(B2) |
申请公布日期 |
2016.08.23 |
申请号 |
US201313963120 |
申请日期 |
2013.08.09 |
申请人 |
University of Tsukuba;Center of Biotechnology of Sfax, University of Sfax, MESRS |
发明人 |
Nakajima Mitsutoshi;Neves Marcos;Isoda Hiroko;Dammak Ilyes;Sayadi Sami |
分类号 |
A61K36/63;A23L1/30 |
主分类号 |
A61K36/63 |
代理机构 |
Locke Lord LLP |
代理人 |
Locke Lord LLP |
主权项 |
1. A method for producing a polyphenol-enriched aqueous solution from olive mill water (OMW) comprising the following sequential steps:
(a) obtaining an OMW after olive oil extraction from olive fruits; (b) pretreating the OMW by removing residual oil, large-sized organic compounds, particles, or soluble molecules from OMW thereby forming a more clear aqueous phase; (c) concentrating the aqueous phase thereby producing a concentrate; (d) mixing the concentrate with at least one solvent thereby producing a mixture; (e) transferring the mixture to a crystallization unit thereby producing a liquid phase and crystals in a solid phase; and (f) distilling the liquid phase in a distillation unit thereby evaporating the solvent and retaining the polyphenol-enriched aqueous solution. |
地址 |
Tsukuba-shi JP |