Microfluidic cartridge for processing and detecting nucleic acids

摘要

A microfluidic cartridge, configured to facilitate processing and detection of nucleic acids, comprising: a top layer comprising a set of cartridge-aligning indentations, a set of sample port-reagent port pairs, a shared fluid port, a vent region, a heating region, and a set of Detection chambers; an intermediate substrate, coupled to the top layer comprising a waste chamber; an elastomeric layer, partially situated on the intermediate substrate; and a set of fluidic pathways, each formed by at least a portion of the top layer and a portion of the elastomeric layer, wherein each fluidic pathway is fluidically coupled to a sample port-reagent port pair, the shared fluid port, and a Detection chamber, comprises a turnabout portion passing through the heating region, and is configured to be occluded upon deformation of the elastomeric layer, to transfer a waste fluid to the waste chamber, and to pass through the vent region.

主权项

1. A cartridge, configured to facilitate processing of a nucleic acid, comprising:
a first layer defining a sample port, a reagent port, and a fluid port; an intermediate substrate coupled to the first layer, wherein the intermediate substrate includes a corrugated surface directly opposing the first layer, wherein the corrugated surface defines a chamber and a set of voids external to the chamber and accessible from a direction perpendicular to a broad surface of the first layer, and wherein at least one of the set of voids of the corrugated surface forms a magnet region configured to receive a magnet; an elastomeric layer positioned between the first layer and the intermediate substrate; and a fluidic pathway formed by at least a portion of the first layer in combination with at least a portion of the elastomeric layer, wherein the fluidic pathway is fluidically coupled to the chamber, the sample port, the reagent port, and the fluid port, and comprises a capture segment configured to cross the magnet region.